ISOLASI DAN KARAKTERISASI LINAMARASE HASIL ISOLASI DARI UMBI SINGKONG (MANIHOT ESCULENTA CRANTZ)

Askurrahman

Jurusan Teknologi Industri Pertanian, Fakultas Pertanian Universitas Trunojoyo
Korespondensi : Jl. Raya Telang PO BOX 2 Kamal-Bangkalan, Email: s_coer@yahoo.com

ABSTRACT

The purpose of this research was to know the optimum condition of linamarase
enzymatic reaction from bitter cassava. These studies are designed in several stages of interrelated. Starting with the determination of cyanide content of cassava tubers, Isolation and characterization linamarase on cassava tuber. The research result showed that linamarase had optimum condition in some activities; pH for about 6, temperature for about 40OC, incubation period for about 3 hours, and Km for about 0.012% and Vmaks for about 0.296 unit.

Key Words: Isolation, Characterization, Linamarase, Cassava (Manihot esculenta Crantz)

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